Anti-PIK3CA antibody produced in rabbit
SIGMA/SAB5700623
Synonym: CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha; PIK3CA
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using PIK3CA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using PIK3CA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HeLa cells, using PIK3CA antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.48 mg/ml |
| form | buffered aqueous solution |
| mol wt | 124 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P42336  |
| General description: | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |