Anti-TNF-α antibody produced in rabbit
SIGMA/SAB5700627
Synonym: DIF; TNF; TNF alpha; TNF-alpha; TNFA; TNFSF2; TNLG1F
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using TNF-α antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.46 mg/ml |
| form | buffered aqueous solution |
| mol wt | 25 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:1000-1:2000 | |
| UniProt accession no. | P01375  |
| General description: | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF (NP_000585.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |