Anti-VCAM1 antibody produced in rabbit
SIGMA/SAB5700628
Synonym: CD106; INCAM-100; VCAM1
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using VCAM1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.14 mg/ml |
| form | buffered aqueous solution |
| mol wt | 71 |
| 74 | |
| 81 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| immunoprecipitation (IP): 1:50-1:200 | |
| western blot: 1:1000-1:3000 | |
| UniProt accession no. | P19320  |
| General description: | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |