Anti-HSP70 antibody produced in rabbit
SIGMA/SAB5700631
Synonym: HEL-S-103; HSP70; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1; HSPA1A
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U2OS cells using HSP70 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.84 mg/ml |
| form | buffered aqueous solution |
| mol wt | 70 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P0DMV8  |
| General description: | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 540 to the C-terminus of human HSP70 (NP_005336.3). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |