Anti-CDKN1B antibody produced in rabbit
SIGMA/SAB5700634
Synonym: CDKN1B; CDKN4; KIP1; MEN1B; MEN4; P27KIP1; p27 KIP 1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using CDKN1B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using CDKN1B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using CDKN1B antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using p27 KIP 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.61 mg/ml |
| form | buffered aqueous solution |
| mol wt | 22 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:20-1:50 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P46527  |
| General description: | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B (NP_004055.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |