Anti-MYCN antibody produced in rabbit
SIGMA/SAB5700642
Synonym: MODED; MYCN; N-myc; NMYC; ODED; bHLHe37
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using MYCN antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.49 mg/ml |
| form | buffered aqueous solution |
| mol wt | 49 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P04198  |
| General description: | This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |