Anti-RIG-I/DDX58 antibody produced in rabbit
SIGMA/SAB5700643
Synonym: DDX58; RIG-I; RIGI; RLR-1; SGMRT2
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human prostate using RIG-I / DDX58 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using RIG-I / DDX58 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using RIG-I / DDX58 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.38 mg/ml |
| form | buffered aqueous solution |
| mol wt | 101 |
| 106 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:20-1:100 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:20-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | O95786  |
| General description: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 830 to the C-terminus of human RIG-I / DDX58 (NP_055129.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |