Anti-Caspase 1 antibody produced in rabbit
SIGMA/SAB5700660
Synonym: CASP1; Caspase 1; ICE; IL1BC; P45; caspase-1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using Caspase-1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using Caspase-1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Confocal immunofluorescence analysis of Raw264.7 cells using Caspase-1 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of Raw264.7 cells using Caspase-1 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of THP-1 cells, using Caspase-1 antibody at 1:1,000 dilution. THP-1 cells were treated by PMA (80 nM) at 37 °C for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 min.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of THP-1 cells, using 3 μg Caspase-1 antibody. Western blot was performed from the immunoprecipitate using Caspase-1 antibody at a dilution of 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.96 mg/ml |
| form | buffered aqueous solution |
| mol wt | 10 |
| 29 | |
| 35 | |
| 42 | |
| 45 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P29466  |
| General description: | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase-1 (NP_150635.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |