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Anti-SNAP25 antibody produced in rabbit

SIGMA/SAB5700664

Synonym: CMS18; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SNAP25; SUP; bA416N4.2; dJ1068F16.2

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5700664-100UL 100 µL
$405.00
1/EA
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Immunofluorescence Immunofluorescence analysis of HeLa cells using SNAP25 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using SNAP25 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 3.49 mg/ml
form buffered aqueous solution
mol wt 23
Quality Level 100 
shipped in wet ice
species reactivity rat, human, mouse
storage temp. −20°C
technique(s) immunofluorescence: 1:20-1:100
  western blot: 1:500-1:2000
UniProt accession no. P60880 
General description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human SNAP25 (NP_003072.2).
Physical form: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage and Stability: Store at -20℃. Avoid freeze / thaw cycles.
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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