Anti-DDIT3/CHOP antibody produced in rabbit
SIGMA/SAB5700686
Synonym: C/EBPzeta; CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; DDIT3 / CHOP; GADD153
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse pancreatic islets using DDIT3 / CHOP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using DDIT3 / CHOP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human thyroid cancer using DDIT3 / CHOP antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using DDIT3 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
Western Blotting Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody at 1:1,000 dilution. Both C2C12 cells and C6 cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.38 mg/ml |
| form | buffered aqueous solution |
| mol wt | 19 |
| 21 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P35638  |
| General description: | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human DDIT3 / CHOP (NP_004074.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |