Anti-mTOR antibody produced in rabbit
SIGMA/SAB5700687
Synonym: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS; mTOR
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human tonsil using mTOR antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using mTOR antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using mTOR antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse liver using mTOR antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using mTOR antibody at dilution of 1:150 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of PC12 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.26 mg/ml |
| form | buffered aqueous solution |
| mol wt | 288 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P42345  |
| General description: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2388-2487 of human mTOR (NP_004949.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |