Anti-ASC/TMS1 antibody produced in rabbit
SIGMA/SAB5700693
Synonym: ASC; ASC / TMS1; CARD5; PYCARD; PYD and CARD domain containing; TMS; TMS-1; TMS1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using ASC / TMS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse liver using ASC / TMS1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of A549 cells using ASC / TMS1 antibody.
Western Blotting Western blot analysis of extracts of various cell lines, using ASC / TMS1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.28 mg/ml |
| form | buffered aqueous solution |
| mol wt | 15 |
| 19 | |
| 21 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q9ULZ3  |
| General description: | This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-195 of human ASC / TMS1 (NP_037390.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |