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Anti-Histone H2AX antibody produced in rabbit

SIGMA/SAB5700699

Synonym: γH2AX; H2A.X; H2A/X; H2AFX; H2AX; Histone H2AX; gamma H2A.X; histone H2AX

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5700699-100UL 100 µL
$405.00
1/EA
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Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using Histone H2AX antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using Histone H2AX antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using Histone H2AX antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Histone H2AX antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 10 s.

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 0.20 mg/ml
form buffered aqueous solution
mol wt 15
Quality Level 100 
shipped in wet ice
species reactivity mouse, human, rat
storage temp. −20°C
technique(s) immunofluorescence: 1:50-1:200
  immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:1000
UniProt accession no. P16104 
General description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Immunogen: Recombinant protein of human Histone H2AX.
Physical form: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage and Stability: Store at -20℃. Avoid freeze / thaw cycles.
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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