Anti-CASP3 antibody produced in rabbit
SIGMA/SAB5700708
Synonym: Active Caspase 3; CASP3; CPP32; CPP32B; Caspase 3; Caspase-3 p12; SCA-1; active Caspase-3; caspase-3
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human mammary cancer using Caspase-3 antibody at dilution of 1:200 (40x lens).
Immunofluorescence Immunofluorescence analysis of Jurkat cells using Caspase-3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25 μM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.55 mg/ml |
| form | buffered aqueous solution |
| mol wt | 31 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| immunohistochemistry: 1:50-1:100 | |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P42574  |
| General description: | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer′s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |