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Anti-CD86 antibody produced in rabbit

SIGMA/SAB5700710

Synonym: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5700710-100UL 100 µL
$405.00
1/EA
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Immunohistochemistry Immunohistochemistry of paraffin-embedded human tonsil using CD86 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of Raw264.7 cells using CD86 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of THP-1 cells, using CD86 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of various cell lines, using CD86 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 120s.

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 2.41 mg/ml
form buffered aqueous solution
mol wt 12
  24
  28
  31
  37
Quality Level 100 
shipped in wet ice
species reactivity rat, human, mouse
storage temp. −20°C
technique(s) immunofluorescence: 1:50-1:200
  immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. P42081 
General description: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).
Physical form: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage and Stability: Store at -20℃. Avoid freeze / thaw cycles.
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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