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Anti-Cox2 antibody produced in rabbit

SIGMA/SAB5700721

Synonym: COX-2; COX2; Cox2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; PTGS2; hCox-2

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SAB5700721-100UL 100 µL
 $405.00
1/EA		 Add To Favorites
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody at dilution of 1:200 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using Cox2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Cox2 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 s.

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 1.19 mg/ml
form buffered aqueous solution
mol wt 68
Quality Level 100 
shipped in wet ice
species reactivity rat, mouse, human
storage temp. −20°C
technique(s) immunofluorescence: 1:50-1:100
  immunohistochemistry: 1:50-1:100
  immunoprecipitation (IP): 1:50-1:100
  western blot: 1:1000-1:4000
UniProt accession no. P35354 
General description: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
Physical form: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage and Stability: Store at -20℃. Avoid freeze / thaw cycles.
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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