Anti-Fibronectin antibody produced in rabbit
SIGMA/SAB5700724
Synonym: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; fibronectin
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat ovary using Fibronectin antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung cancer using Fibronectin antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using Fibronectin antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse leydig cells using Fibronectin antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Fibronectin antibody at 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.05 mg/ml |
| form | buffered aqueous solution |
| mol wt | 221-272 |
| 71-73 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P02751  |
| General description: | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin (NP_002017.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |