Anti-BDNF antibody produced in rabbit
SIGMA/SAB5700726
Synonym: ANON2; BDNF; BULN2
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon carcinoma using BDNF antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human breast cancer using BDNF antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using BDNF antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of U2OS cells using BDNF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-251MG cells using BDNF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using BDNF antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.51 mg/ml |
| form | buffered aqueous solution |
| mol wt | 27 |
| 28 | |
| 29 | |
| 31 | |
| 37 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P23560  |
| General description: | This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer′s, Parkinson′s, and Huntington′s disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (NP_733928.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |