Anti-CASC5 antibody produced in rabbit
SIGMA/SAB5700728
Synonym: AF15Q14; CASC5; CT29; D40; KNL1; MCPH4; PPP1R55; Spc7; hKNL-1; hSpc105
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using CASC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using CASC5 antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using CASC5 antibody at 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.01 mg/ml |
| form | buffered aqueous solution |
| mol wt | 195 |
| 205 | |
| 262 | |
| 265 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q8NG31  |
| General description: | The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |