Anti-SQSTM1/p62 antibody produced in rabbit
SIGMA/SAB5700845
Synonym: A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; SQSTM1; ZIP3; p60; p62; p62B
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using SQSTM1 / p62 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Confocal immunofluorescence analysis of HeLa cells using SQSTM1 / p62 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Confocal immunofluorescence analysis of U-2 OS cells using SQSTM1 / p62 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U2OS cells using SQSTM1 / p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 using SQSTM1 / p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SQSTM1 / p62 antibody. Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody at a dilution of 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.14 mg/ml |
| form | buffered aqueous solution |
| mol wt | 38 |
| 47 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q13501  |
| General description: | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |