Anti-METTL3 antibody produced in rabbit
SIGMA/SAB5700853
Synonym: IME4; M6A; METTL3; MT-A70; Spo8; hMETTL3
Product Type: Chemical
Immunofluorescence Confocal immunofluorescence analysis of HeLa cells using METTL3 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Confocal immunofluorescence analysis of U-2 OS cells using METTL3 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of C6 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using METTL3 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 1 s.
Immunoprecipitation Immunoprecipitation analysis of 150 μg extracts of 293T cells using 3 μg METTL3 antibody. Western blot was performed from the immunoprecipitate using METTL3 antibody at a dilution of 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 5.81 mg/ml |
| form | buffered aqueous solution |
| mol wt | 25 |
| 64 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:20-1:50 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q86U44  |
| General description: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransf |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |