Anti- HK1 antibody produced in rabbit
SIGMA/SAB5700876
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using HK1 Rabbit mAb (SAB5700876) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human thyroid cancer using HK1 Rabbit mAb (SAB5700876) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using HK1 Rabbit mAb (SAB5700876) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using HK1 Rabbit mAb (SAB5700876) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts from normal (control) and HK1 knockout (KO) 293T cells, using HK1 Rabbit mAb (SAB5700876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
Western Blotting Western blot analysis of extracts of various cell lines, using HK1 Rabbit mAb (SAB5700876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
Immunoprecipitation Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg HK1 antibody (SAB5700876). Western blot was performed from the immunoprecipitate using HK1 (SAB5700876) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.65 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P19367  |
| General description: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016] |
| Immunogen: | A synthesized peptide derived from human Hexokinase 1 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |