Anti-GluR2/GRIA2 antibody produced in rabbit

SIGMA/SAB5700955

Product Type: Chemical

Catalog Number	Price


45-SAB5700955-100UL	$405.00 1/EA	Add To Favorites

Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using GluR2/GRIA2 Rabbit mAb (SAB5700955) at dilution of 1:100 (40x lens).

Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using GluR2/GRIA2 Rabbit mAb (SAB5700955) at dilution of 1:100 (40x lens).

Immunofluorescence Immunofluorescence analysis of SH-SY5Y cells using GluR2/GRIA2 Rabbit mAb (SAB5700955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence Immunofluorescence analysis of rat brain using GluR2/GRIA2 Rabbit mAb (SAB5700955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence Immunofluorescence analysis of mouse brain using GluR2/GRIA2 Rabbit mAb (SAB5700955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blotting Western blot analysis of extracts of various cell lines, using GluR2/GRIA2 Rabbit mAb (SAB5700955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.

Properties
Descriptions
Basic Data

antibody product type	primary antibodies
biological source	rabbit
clone	monoclonal
concentration	1.3 mg/mL
form	liquid
Quality Level	100
shipped in	wet ice
species reactivity	mouse, rat, human
storage temp.	−20°C
technique(s)	immunohistochemistry: 1:50-1:200
	western blot: 1:500-1:2000
UniProt accession no.	P42262

General description:	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]
Immunogen:	A synthesized peptide derived from human GluR2/GRIA2
Physical form:	Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Storage Temp.

−20°C

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