Anti-MAD2/MAD2L1 antibody produced in rabbit
SIGMA/SAB5700978
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat spleen using MAD2/MAD2/MAD2L1 Rabbit mAb (SAB5700978) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using MAD2/MAD2/MAD2L1 Rabbit mAb (SAB5700978) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using MAD2/MAD2/MAD2L1 Rabbit mAb (SAB5700978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.22 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q13257  |
| General description: | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human MAD2/MAD2/MAD2L1 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |