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Anti-CD127/IL7R antibody produced in rabbit

SIGMA/SAB5701010

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5701010-100UL
$405.00
1/EA
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Immunofluorescence Immunofluorescence analysis of K562 cells using CD127/IL7R Rabbit mAb (SAB5701010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using CD127/IL7R Rabbit mAb (SAB5701010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.

 

antibody product type primary antibodies
biological source rabbit
clone monoclonal
concentration 1.34 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity human, rat, mouse
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. P16871 
General description: The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]
Immunogen: A synthesized peptide derived from human CD127/IL7R
Physical form: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage Temp. −20°C

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