Anti-NUR77 antibody produced in rabbit
SIGMA/SAB5701019
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using NUR77 Rabbit mAb (SAB5701019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of HepG2 cells using 3 μg NUR77 antibody (SAB5701019). Western blot was performed from the immunoprecipitate using NUR77 antibody (SAB5701019) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.53 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P22736  |
| General description: | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| Immunogen: | A synthesized peptide derived from human NUR77 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |