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Anti-RAB7 antibody produced in rabbit

SIGMA/SAB5701027

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5701027-100UL
$405.00
1/EA
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Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using RAB7 Rabbit mAb (SAB5701027) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using RAB7 Rabbit mAb (SAB5701027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.

 

antibody product type primary antibodies
biological source rabbit
clone monoclonal
concentration 0.3 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity mouse, human, rat
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. P51149 
General description: RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]
Immunogen: A synthesized peptide derived from human RAB7
Physical form: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage Temp. −20°C

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