Anti-Ku80 antibody produced in rabbit
SIGMA/SAB5701032
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using Ku80 Rabbit mAb (SAB5701032) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using Ku80 Rabbit mAb (SAB5701032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Ku80 Rabbit mAb (SAB5701032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Ku80 antibody (SAB5701032). Western blot was performed from the immunoprecipitate using Ku80 antibody (SAB5701032) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.11 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P13010  |
| General description: | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Ku80 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |