Anti-PCNA antibody produced in rabbit
SIGMA/SAB5701035
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat liver using PCNA Rabbit mAb (SAB5701035) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human appendix using PCNA Rabbit mAb (SAB5701035) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using PCNA Rabbit mAb (SAB5701035) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using PCNA Rabbit mAb (SAB5701035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg PCNA antibody (SAB5701035). Western blot was performed from the immunoprecipitate using PCNA antibody (SAB5701035) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.18 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P12004  |
| General description: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human PCNA |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |