Anti- CD168/RHAMM antibody produced in rabbit
SIGMA/SAB5701037
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of U2OS cells using CD168/RHAMM antibody (SAB5701037). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using CD168/RHAMM antibody (SAB5701037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blotting Western blot analysis of extracts from normal (control) and CD168/RHAMM Rabbit pAb knockout (KO) HeLa cells, using CD168/RHAMM Rabbit pAb antibody (SAB5701037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.95 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | O75330  |
| General description: | The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |