Anti- IDH1 antibody produced in rabbit
SIGMA/SAB5701050
Product Type: Chemical
antibody product type | primary antibodies |
biological source | rabbit |
clone | polyclonal |
concentration | 1.83 mg/mL |
form | liquid |
Quality Level | 100 ![]() |
shipped in | wet ice |
species reactivity | rat, human, mouse |
storage temp. | −20°C |
technique(s) | immunohistochemistry: 1:50-1:200 |
western blot: 1:500-1:2000 | |
UniProt accession no. | O75874 ![]() ![]() |
General description: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2). |
Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage Temp. | −20°C |