Anti- NF2 antibody produced in rabbit
SIGMA/SAB5701057
Product Type: Chemical
Western Blotting Western blot analysis of extracts of HepG2 cells, using NF2 Rabbit pAb (SAB5701057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 s.
Western Blotting Western blot analysis of extracts from normal (control) and NF2 Rabbit pAb knockout (KO) HeLa cells, using NF2 Rabbit pAb antibody (SAB5701057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of A-549 cells using 3 μg NF2 antibody (SAB5701057). Western blot was performed from the immunoprecipitate using NF2 antibody (SAB5701057) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 6.49 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P35240  |
| General description: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |