Anti-KLF4 antibody produced in rabbit
SIGMA/SAB5701058
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using KLF4 Rabbit mAb (SAB5701058) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using KLF4 Rabbit mAb (SAB5701058) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spinal cord using KLF4 Rabbit mAb (SAB5701058) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using KLF4 Rabbit mAb (SAB5701058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using KLF4 Rabbit mAb (SAB5701058) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using KLF4 Rabbit mAb (SAB5701058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
Western Blotting Western blot analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (SAB5701058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KLF4 antibody (SAB5701058). Western blot was performed from the immunoprecipitate using KLF4 antibody (SAB5701058) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1.2 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | O43474  |
| General description: | This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
| Immunogen: | A synthesized peptide derived from human KLF4 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |