Anti- Twist antibody produced in rabbit
SIGMA/SAB5701071
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat liver using Twist Rabbit pAb (SAB5701071) at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal cancer using Twist Rabbit pAb (SAB5701071) at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse lung using Twist Rabbit pAb (SAB5701071) at dilution of 1:200 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Twist antibody (SAB5701071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
Western Blotting Western blot analysis of extracts from normal (control) and Twist knockout (KO) 293T cells, using Twist antibody (SAB5701071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.63 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q15672  |
| General description: | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |