Anti-CDC20 antibody produced in rabbit
SIGMA/SAB5701074
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded Rat ovary using CDC20 antibody (SAB5701074) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Rat spleen using CDC20 antibody (SAB5701074) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human thyroid cancer using CDC20 antibody (SAB5701074) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using CDC20 antibody (SAB5701074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of LO2 cells using 3 μg CDC20 antibody (SAB5701074). Western blot was performed from the immunoprecipitate using CDC20 antibody (SAB5701074) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.29 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q12834  |
| General description: | CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |