Anti-SIN3A antibody produced in rabbit
SIGMA/SAB5701075
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of U2OS cells using Sin3A antibody (SAB5701075). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Sin3A antibody (SAB5701075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Sin3A antibody (SAB5701075). Western blot was performed from the immunoprecipitate using Sin3A antibody (SAB5701075) at a dilition of 1:500.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.48 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q96ST3  |
| General description: | The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Sin3A (NP_056292.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |