Anti- Acetyl-HMGB1-K29 antibody produced in rabbit
SIGMA/SAB5701077
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of C6 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (SAB5701077) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (SAB5701077) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using [KO Validated] Acetyl-HMGB1-K29 Rabbit pAb (SAB5701077) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts from normal (control) and Acetyl-HMGB1-K29 knockout (KO) 293T cells, using Acetyl-HMGB1-K29 antibody (SAB5701077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 5 min.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.93 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P09429  |
| General description: | This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Immunogen: | A synthetic acetylated peptide around K29 of human HMGB1. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |