Anti- HIF1α antibody produced in rabbit
SIGMA/SAB5701087
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat ovary using HIF1α Rabbit pAb (SAB5701087) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using HIF1α Rabbit pAb (SAB5701087) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human breast cancer using HIF1α Rabbit pAb (SAB5701087) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts from normal (control) and HIF1α knockout (KO) HeLa cells, using HIF1α antibody (SAB5701087) at 1:1000 dilution.Both Hela cells and HIF1α knockout (KO) Hela cells were treated by Cobalt chloride (250 μM) at 37 °C for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 180 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.23 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q16665  |
| General description: | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
| Immunogen: | Recombinant protein of human HIF1α. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |