Anti- LRRC59 antibody produced in rabbit
SIGMA/SAB5701094
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using LRRC59 Rabbit pAb (SAB5701094) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using LRRC59 antibody (SAB5701094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts from normal (control) and LRRC59 knockout (KO) 293T cells, using LRRC59 antibody (SAB5701094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.45 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q96AG4  |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human LRRC59 (NP_060979.2). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |