Anti-PN1 antibody produced in rabbit
SIGMA/SAB5701097
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using PABPN1 Rabbit mAb (SAB5701097) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (SAB5701097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.19 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q86U42  |
| General description: | This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. [provided by RefSeq, Dec 2010] |
| Immunogen: | A synthesized peptide derived from human PABPN1 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |