Anti-MAP1LC3B antibody produced in mouse
SIGMA/SAB5701099
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of 293T cells using MAP1LC3B Mouse mAb (SAB5701099) at dilution of 1:100 (40x lens). 293T cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of C6 cells using MAP1LC3B Mouse mAb (SAB5701099) at dilution of 1:100 (40x lens).C6 cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 treated by Chloroquine and 293T cells using MAP1LC3B Mouse mAb (SAB5701099) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody (SAB5701099) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 180 s.
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | monoclonal |
| concentration | 2.86 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q9GZQ8  |
| General description: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| Immunogen: | Recombinant protein of human MAP1LC3B. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |