Anti-CDX2 antibody produced in rabbit
SIGMA/SAB5701170
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat rectum using CDX2 Rabbit mAb (SAB5701170) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using CDX2 Rabbit mAb (SAB5701170) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse colon using CDX2 Rabbit mAb (SAB5701170) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of mouse colon using CDX2 Rabbit mAb (SAB5701170) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using CDX2 antibody (SAB5701170) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
Immunoprecipitation Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg CDX2 antibody (SAB5701170). Western blot was performed from the immunoprecipitate using CDX2 antibody (SAB5701170) at a dilition of 1:500.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.53 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q99626  |
| General description: | This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012] |
| Immunogen: | A synthesized peptide derived from human CDX2 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |