Anti-Glucosylceramidase beta (GBA) antibody produced in rabbit
SIGMA/SAB5701181
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver using Glucosylceramidase beta (Glucosylceramidase beta (GBA)) Rabbit mAb (SAB5701181) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase beta (GBA)) antibody (SAB5701181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of Rat brain, using Glucosylceramidase beta (Glucosylceramidase beta (GBA)) antibody (SAB5701181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.51 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P04062  |
| General description: | This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] |
| Immunogen: | A synthesized peptide derived from human Glucosylceramidase beta (Glucosylceramidase beta (GBA)) |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |