Anti-Heme Oxygenase 1 (HO-1/HMOX1) antibody produced in rabbit
SIGMA/SAB5701183
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human kidney cancer using Heme Oxygenase 1 (HO-1/HMOX1) (HO-1/HMOX1) Rabbit mAb (SAB5701183) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse liver using Heme Oxygenase 1 (HO-1/HMOX1) (HO-1/HMOX1) Rabbit mAb (SAB5701183) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Heme Oxygenase 1 (HO-1/HMOX1) (HO-1/HMOX1) antibody (SAB5701183) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.48 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P09601  |
| General description: | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Heme Oxygenase 1 (HO-1/HMOX1) (HO-1/HMOX1) |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |