Anti- METTL3 antibody produced in rabbit
SIGMA/SAB5701186
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of HeLa cells using METTL3 Rabbit mAb (SAB5701186) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using METTL3 antibody (SAB5701186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 3 min.
Western Blotting Western blot analysis of extracts from normal (control) and METTL3 knockout (KO) 293T cells, using METTL3 antibody (SAB5701186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 3 min.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.5 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q86U44  |
| General description: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransf |
| Immunogen: | A synthesized peptide derived from human METTL3 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |