Anti- Src antibody produced in rabbit
SIGMA/SAB5701207
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Src Rabbit mAb (SAB5701207) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Src antibody (SAB5701207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts from normal (control) and Src knockout (KO) HeLa cells, using Src antibody (SAB5701207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 min.
Western Blotting Western blot analysis of extracts of Jurkat cells, using Src antibody (SAB5701207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 min.
Immunoprecipitation Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg Src antibody (SAB5701207). Western blot was performed from the immunoprecipitate using Src antibody (SAB5701207) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.62 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P12931  |
| General description: | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Src |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |