Anti-TDP-43/TARDB antibody produced in rabbit
SIGMA/SAB5701208
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using TDP-43/TARDB Rabbit mAb (SAB5701208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using TDP-43/TARDB antibody (SAB5701208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TDP-43/TARDB antibody (SAB5701208). Western blot was performed from the immunoprecipitate using TDP-43/TARDB antibody (SAB5701208) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.93 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q13148  |
| General description: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human TDP-43/TARDB |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |