Anti- PD-L1/CD274 antibody produced in rabbit
SIGMA/SAB5701213
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PD-L1/CD274 Rabbit mAb (SAB5701213) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts from normal (control) and PD-L1/CD274 knockout (KO) A-549 cells, using PD-L1/CD274 antibody (SAB5701213) at 1:1000 dilution.Both A-549 cells and PD-L1/CD274 knockout (KO) A-549 cells were treated by IFNG (100 ng/mL) at 37 °C for 48 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q9NZQ7  |
| General description: | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide of human PD-L1/CD274. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |