Anti- Lamin A/C antibody produced in rabbit
SIGMA/SAB5701253
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody (SAB5701253) at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (SAB5701253) at dilution of 1:400 - 1:3200 (40x lens).
Immunofluorescence Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody (SAB5701253) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of L929 cells using Lamin A/C antibody (SAB5701253) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody (SAB5701253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U2OS cells using Lamin A/C antibody (SAB5701253) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody (SAB5701253) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (SAB5701253) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (SAB5701253) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (SAB5701253) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of K-562 cells using 3 μg Lamin A/C antibody (SAB5701253). Western blot was performed from the immunoprecipitate using Lamin A/C antibody (SAB5701253) at a dilition of 1:20000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1.2 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:1000 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:50000-1:200000 | |
| UniProt accession no. | P02545  |
| General description: | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. |
| Immunogen: | Recombinant protein of human Lamin A/C. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |