Anti-Acetyl-Histone H4-K5 antibody produced in rabbit
SIGMA/SAB5701254
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K5 antibody (SAB5701254) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Acetyl-Histone H4-K5 antibody (SAB5701254) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using Acetyl-Histone H4-K5 antibody (SAB5701254) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K5 antibody (SAB5701254).C6 cells were treated by TSA (1 μM) at 37 °C for 18 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Acetyl-Histone H4-K5 antibody (SAB5701254).NIH-3T3 cells were treated by TSA (1 μM) at 37 °C for 18 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K5 antibody (SAB5701254).U-2 OS cells were treated by TSA (1 μM) at 37 °C for 18 hours(left). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (SAB5701254) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Chromatin Immunoprecipitation Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (SAB5701254) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.08 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P62805  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015] |
| Immunogen: | A synthesized peptide derived from human Histone H4 (acetyl K5). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |